Serum paraoxonase-3 concentration in HIV-infected patients. Evidence for a protective role against oxidation.

نویسندگان

  • Gerard Aragonès
  • Anabel García-Heredia
  • Marta Guardiola
  • Anna Rull
  • Raúl Beltrán-Debón
  • Judit Marsillach
  • Carlos Alonso-Villaverde
  • Bharti Mackness
  • Michael Mackness
  • Juan Pedro-Botet
  • Pedro Pardo-Reche
  • Jorge Joven
  • Jordi Camps
چکیده

We investigated the influence of the HIV infection on serum paraoxonase-3 (PON3) concentration and assessed the relationships with lipoprotein-associated abnormalities, immunological response, and accelerated atherosclerosis. We studied 207 HIV-infected patients and 385 healthy volunteers. Serum PON3 was determined by in-house ELISA, and PON3 distribution in lipoproteins was investigated by fast-performance liquid chromatography (FPLC). Polymorphisms of the PON3 promoter were analyzed by the Iplex Gold MassArray(TM) method. PON3 concentrations were increased (about three times) in HIV-infected patients with respect to controls (P < 0.001) and were inversely correlated with oxidized LDL levels (P = 0.038). Long-term use of nonnucleoside reverse transcriptase inhibitor (NNRTI)-based antiretroviral therapy was associated with a decrease of PON3 concentrations. In a multivariate linear regression analysis, these relationships were still strong when the main confounding covariates were considered. PON3 was mainly found in HDL in HIV-infected patients, but a substantial amount of the protein was detected in LDL particles. This study reports for the first time an important increase in serum PON3 concentrations in HIV-infected patients that is associated with their oxidative status and their treatment with NNRTI. Long-term, prospective studies are needed to confirm the possible influence of this enzyme on the course of this disease and its possible utility as an analytical biomarker.

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عنوان ژورنال:
  • Journal of lipid research

دوره 53 1  شماره 

صفحات  -

تاریخ انتشار 2012