Serum paraoxonase-3 concentration in HIV-infected patients. Evidence for a protective role against oxidation.
نویسندگان
چکیده
We investigated the influence of the HIV infection on serum paraoxonase-3 (PON3) concentration and assessed the relationships with lipoprotein-associated abnormalities, immunological response, and accelerated atherosclerosis. We studied 207 HIV-infected patients and 385 healthy volunteers. Serum PON3 was determined by in-house ELISA, and PON3 distribution in lipoproteins was investigated by fast-performance liquid chromatography (FPLC). Polymorphisms of the PON3 promoter were analyzed by the Iplex Gold MassArray(TM) method. PON3 concentrations were increased (about three times) in HIV-infected patients with respect to controls (P < 0.001) and were inversely correlated with oxidized LDL levels (P = 0.038). Long-term use of nonnucleoside reverse transcriptase inhibitor (NNRTI)-based antiretroviral therapy was associated with a decrease of PON3 concentrations. In a multivariate linear regression analysis, these relationships were still strong when the main confounding covariates were considered. PON3 was mainly found in HDL in HIV-infected patients, but a substantial amount of the protein was detected in LDL particles. This study reports for the first time an important increase in serum PON3 concentrations in HIV-infected patients that is associated with their oxidative status and their treatment with NNRTI. Long-term, prospective studies are needed to confirm the possible influence of this enzyme on the course of this disease and its possible utility as an analytical biomarker.
منابع مشابه
Tetanus and Diphtheria Seroprevalence in Patients Infected With Human Immunodeficiency Virus
Background and Objectives: HIV infected patients are at risk for vaccine-preventable infections such as tetanus and diphtheria (Td). In these patients, these antibodies tend to decline faster. Due to the limited data, this study assessed the seroprevalence of tetanus and diphtheria antibodies in HIV infected patients in Tehran, Iran. Materials and Methods: This case-control study was carried o...
متن کاملParaoxonase role in dyslipidemia in thyroid disease (review article)
Introduction: Thyroid disease e.g. hypothyroidism, hyperthyroidism, and subclinical hypothyroidism are among the most common endocrine disorders. Dyslipidemia is prevalent in thyroid diseases and lipid oxidation due to oxidative stress play an important role in the pathogenesis of thyroid diseases. Antioxidant enzymes such as paraxonase have a protective role in these patients and their activit...
متن کاملEffect of Pomegranate Juice on Paraoxonase Enzyme Activity in Patients with Type 2 Diabetes
OBJECTIVE: The aim of this study was to investigate the effect of pomegranate juice (PJ) on the paraoxonase and arylesterase activity of human serum paraoxonase( PON1). MATERIAL AND METHOD: Fifty patients with type 2 diabetes mellitus consumed 200 ml of PJ daily for a period of 6 weeks. Blood was collected from the patients before and after PJ consumption after 12 h of fasting. Blood sugar, to...
متن کاملPlasma paraoxonase, sphingosine-1-phosphate, total sialic acid, and heat shock protein-27 in the liver of the sheep naturally infected with Cysticercus tenuicollis: Evidence on pathological changes
The present study aimed to investigate whether sphingosine 1 phosphate (S1P), paraoxonase (PON), total sialic acid (TSA), and heat shock protein-27 (HSP27) are altered in the sheep during infection of the liver with Cysticercus tenuicollis. This study was conducted on40 healthy sheep and40 sheep with Cysticercus tenuicollis infection. The infected and non-infected animals were selected based on...
متن کاملImmune Response to Standard Hepatitis B Vaccination in HIV-Infected Patients
Background: Due to their similar routes of transmission, human immunodeficiency virus (HIV) and hepatitis B virus (HBV) co-infection occurs considerably. HBV infection progresses more rapidly in HIV-infected patients. Therefore, HBV vaccination of all non-immune HIV infected patients is recommended. On the other hand, HIV-infected subjects have suboptimal responses to HBV vaccine. In this study...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of lipid research
دوره 53 1 شماره
صفحات -
تاریخ انتشار 2012